| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116039 |
| Chromosome: | chromosome 12 |
| Location: | 7287929 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557700 | (1 of 1) K01069 - hydroxyacylglutathione hydrolase (E3.1.2.6, gloB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTTAATTACATTTTCGTGTGACTGCGG |
| Internal bar code: | CAACCTTAATTTTGGCATGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 88.739 |
| LEAP-Seq n confirming: | 1316 |
| LEAP-Seq n nonconfirming: | 167 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGCAGAATGCAGAATGG |
| Suggested primer 2: | TATATTGGCGATGTGGGAGG |