| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.116055 |
| Chromosome: | chromosome 5 |
| Location: | 2078460 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234600 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTCCCTCTGCAGCTGTGCCCGCTGCTG |
| Internal bar code: | GGGCGGGTGTGGCGTTCGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 348 |
| LEAP-Seq percent confirming: | 31.7986 |
| LEAP-Seq n confirming: | 1105 |
| LEAP-Seq n nonconfirming: | 2370 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACCAGCTCGTCCTCATCAT |
| Suggested primer 2: | AGCTCGAGTCCTCTTGCTTG |