Insertion junction: LMJ.RY0402.116703_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.116703
Chromosome:chromosome 10
Location:4466438
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g451600 SRR28,SRR28B (1 of 1) PF00059//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4); Lectin-domain glycosyl hydrolase intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGATTTCGTGTGGAAGAGTCGGGTTTGATC
Internal bar code:AGGTTTCTCTCCCGATCCGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1203
LEAP-Seq percent confirming:99.6934
LEAP-Seq n confirming:2276
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTCAATGGGTCACAGTGC
Suggested primer 2:GGAGTCCCAGTGGAAGTTGA