| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.117016 |
| Chromosome: | chromosome 3 |
| Location: | 4557032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176833 | NAP1,NAP,DII5 | (1 of 7) PF00022 - Actin (Actin); Novel actin protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACTTGCGCTCCGGCGGCGCCACCACGC |
| Internal bar code: | CTCGTACTGCAAATAGCAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 188 |
| LEAP-Seq percent confirming: | 98.3607 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGTCGGCAGCCTCTCAAC |
| Suggested primer 2: | AATCCCACACATCCCTACCA |