Insertion junction: LMJ.RY0402.117030_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.117030
Chromosome:chromosome 1
Location:2326111
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g012700 KCN6 (1 of 4) IPR000595//IPR002110//IPR005821//IPR018490//IPR020683 - Cyclic nucleotide-binding domain // Ankyrin repeat // Ion transport domain // Cyclic nucleotide-binding-like // Ankyrin repeat-containing domain; Voltage-gated potassium channel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCCACAGGAGAAGCAGGATGACGACGA
Internal bar code:GTGACAATGGGAGAGCGGCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:316
LEAP-Seq percent confirming:97.4138
LEAP-Seq n confirming:113
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCTCTCAACGGCTGAA
Suggested primer 2:ACGCACGCAATTCCTAAAAC