| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.117139 |
| Chromosome: | chromosome 9 |
| Location: | 5895517 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402515 | AMX1 | (1 of 2) 1.4.3.21 - Primary-amine oxidase / Copper amine oxidase; Copper amine oxidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCCAAGGTCGGGCTTGCATGGCCTCTG |
| Internal bar code: | ATACTACAGCGTCTACCTCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.5067 |
| LEAP-Seq n confirming: | 10086 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGTCGGCTTTTTCATAG |
| Suggested primer 2: | CGTTGAAAGCAGTGGCTACA |