Mutant strain: LMJ.RY0402.117139

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.117139_1 Cre01.g038151 (1 of 1) PTHR23069:SF0 - TAT-BINDING HOMOLOG 7 intron 58
LMJ.RY0402.117139_2 Cre04.g216700 PHOX,PHO5 Phosphate-repressible alkaline phosphatase; (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase intron 58
LMJ.RY0402.117139_3 Cre09.g402515 AMX1 (1 of 2) 1.4.3.21 - Primary-amine oxidase / Copper amine oxidase; Copper amine oxidase 5'UTR 95
LMJ.RY0402.117139_4 Cre09.g402515 AMX1 (1 of 2) 1.4.3.21 - Primary-amine oxidase / Copper amine oxidase; Copper amine oxidase 5'UTR 95

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Cisplatin, screen 2 of 4 (No phenotype detected)
Cisplatin, screen 4 of 4 (No phenotype detected)
High temperature, screen 5 of 7 (~10.0% growth defect)
Hydroxyurea, screen 1 of 2 (~20.0% growth defect)
Paromomycin, screen 3 of 6 (~10.0% improved growth)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.