Insertion junction: LMJ.RY0402.117186_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.117186
Chromosome:chromosome 4
Location:675341
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCCCATGTGGCAAGCAATGAGAAGAAGA
Internal bar code:ATGTCTTTTTTCGACGTGCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:688
LEAP-Seq percent confirming:99.543
LEAP-Seq n confirming:1089
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCAAAGCTAACCGCGTC
Suggested primer 2:TTTGCAACAAGCTCATCGAC