| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117239 |
| Chromosome: | chromosome 7 |
| Location: | 2458885 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329150 | SPL21,SPLH1 | DEAH-box helicase, possible nuclear pre-mRNA splicing factor, N-terminal; (1 of 3) K12818 - ATP-dependent RNA helicase DHX8/PRP22 (DHX8, PRP22) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCTGGTGCATCTGTATGGGAATGGGAC |
| Internal bar code: | GTGTGGGGCAGGCACGCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 99.7854 |
| LEAP-Seq n confirming: | 26500 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAACCCTACACTCGGCAC |
| Suggested primer 2: | GGTAACTGGCAGGTATGCGT |