| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.117395 |
| Chromosome: | chromosome 12 |
| Location: | 1172541 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489900 | CYG54 | (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCTGTTGCAGGACAATGGTGGCGAGTA |
| Internal bar code: | CCTCGGTGCTGTTGGTAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 861 |
| LEAP-Seq percent confirming: | 98.2278 |
| LEAP-Seq n confirming: | 4545 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAACGGTTCGGGCTTATCA |
| Suggested primer 2: | GTGGATGTAGTTCTGCCGGT |