Insertion junction: LMJ.RY0402.118531_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118531
Chromosome:chromosome 17
Location:3872087
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g728250 (1 of 1) PF16908//PF16910 - Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Repeating coiled region of VPS13 (VPS13_mid_rpt) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACGTACCCGTTGCCGTACGACTGGCTG
Internal bar code:GGTTGGTACTACTCTGCCTATT

Confirmation - LEAP-Seq

LEAP-Seq distance:305
LEAP-Seq percent confirming:99.7246
LEAP-Seq n confirming:4345
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGCACACACACGTACAA
Suggested primer 2:GCCTTGCCTTCTGTTCTGAC