Insertion junction: LMJ.RY0402.118749_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.118749
Chromosome:chromosome 14
Location:3731869
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632050 POB25,RPG1,RPGRIP1 Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCTCGCAATGCGTTGCCGCACATACGC
Internal bar code:CAATTATCCGGTGGTCCAGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:345
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCTGTAACCTTGCAACCC
Suggested primer 2:ACACACACACACACATCCCC