| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119034 |
| Chromosome: | chromosome 9 |
| Location: | 3574393 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390060 | RAA4 | psaA mRNA trans-splicing factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCACCGGAAGGACTGGCAGGAGCTCAC |
| Internal bar code: | CTCATACGTACCTTTAAAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 407 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCAGCTGCTGTGTGTTT |
| Suggested primer 2: | GGTGCAGGGCAACTATGAAT |