| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119192 |
| Chromosome: | chromosome 16 |
| Location: | 2994623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664550 | SHMT1 | (1 of 1) PTHR11680//PTHR11680:SF10 - SERINE HYDROXYMETHYLTRANSFERASE // SERINE HYDROXYMETHYLTRANSFERASE 1, MITOCHONDRIAL; Serine hydroxymethyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCACTGAAGCGCGATCCCGATCACACA |
| Internal bar code: | GATTACGCGATCGTCCTTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 60.3175 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTACTTGGGTGCGAAAT |
| Suggested primer 2: | TGAGCTCATGCTGTTCTGCT |