| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119555 |
| Chromosome: | chromosome 12 |
| Location: | 7637182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554800 | PRK1 | (1 of 1) 2.7.1.19 - Phosphoribulokinase / Phosphopentokinase; Phosphoribulokinase, chloroplastic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTGGCCGTACCAGGTGTCGGGGCCGT |
| Internal bar code: | CATCGCTCGGCGTTGCGAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 358 |
| LEAP-Seq percent confirming: | 99.6702 |
| LEAP-Seq n confirming: | 1209 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATAGGACGTGTTCGGG |
| Suggested primer 2: | AGGTCGTCCACCACAGCTAC |