| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119572 |
| Chromosome: | chromosome 8 |
| Location: | 4105929 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379650 | TIC20 | 20 kDa translocon at the inner membrane of chloroplasts | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACCCTCGGTCAAGACCGCAGAGTCCGG |
| Internal bar code: | GTCTATTTTCGAGCCTCCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 99.6587 |
| LEAP-Seq n confirming: | 876 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCAATTGAGCGACCTTGG |
| Suggested primer 2: | GCTTGGGTAGCAGTGGATGT |