| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119684 |
| Chromosome: | chromosome 11 |
| Location: | 682553 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467622 | (1 of 1) 1.1.1.317 - Perakine reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGCACGTCGGGATGATCTCGTCCTTA |
| Internal bar code: | CCATGTCACGTGTTCTTATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 98.9311 |
| LEAP-Seq n confirming: | 6664 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTGCGGGATTTAGATT |
| Suggested primer 2: | GGGTGATATGAAACATGGGC |