| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119684 |
| Chromosome: | chromosome 11 |
| Location: | 682659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467622 | (1 of 1) 1.1.1.317 - Perakine reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGCAGTGGGATCCTGAGCCGCCACGC |
| Internal bar code: | AGGGATATGGGGTGCCGAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 5.89803 |
| LEAP-Seq n confirming: | 177 |
| LEAP-Seq n nonconfirming: | 2824 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTTGCGGGATTTAGATT |
| Suggested primer 2: | GGGTGATATGAAACATGGGC |