Insertion junction: LMJ.RY0402.119818_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119818
Chromosome:chromosome 14
Location:3736137
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g632050 POB25,RPG1,RPGRIP1 Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGAGGCCAAGCGGCGAGCAGGCTTCACA
Internal bar code:GGGTATCGACATGCAGGTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:740
LEAP-Seq percent confirming:97.7408
LEAP-Seq n confirming:3461
LEAP-Seq n nonconfirming:80
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGTCACACCAGACCACAC
Suggested primer 2:GTGGAAGGTTTGTTGGGTTG