| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120188 |
| Chromosome: | chromosome 11 |
| Location: | 3626549 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482150 | (1 of 100) IPR001810 - F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTTATGGCGGTTATGGTGGTTATGG |
| Internal bar code: | TTGGACCGACTTAGACGCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 77 |
| LEAP-Seq percent confirming: | 99.9121 |
| LEAP-Seq n confirming: | 5683 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGCAACGTTTTTGTCC |
| Suggested primer 2: | TTGGGGTTAGGAGACACAGG |