| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120237 |
| Chromosome: | chromosome 9 |
| Location: | 3508496 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389763 | HPAT4 | Hydroxyproline O-arabinosyl transferase 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGAGGTCGGGCAGCTGCTTGATGGCCTC |
| Internal bar code: | AGGACGTACGTGGGTAGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 689 |
| LEAP-Seq percent confirming: | 99.4062 |
| LEAP-Seq n confirming: | 2009 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTGTAACACTCGCCTGC |
| Suggested primer 2: | CAGTTCCAGACAGGCAGACA |