Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.120954 |
Chromosome: | chromosome_12 |
Location: | 3048989 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g500550 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGAAACCGCTGGCTAGCAGAGTCCAGTGG |
Internal bar code: | TGGCGGGTCCGGTCGATGGGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 817 |
LEAP-Seq percent confirming: | 99.8088 |
LEAP-Seq n confirming: | 1044 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGCTGCTGGTAATGGAAT |
Suggested primer 2: | GTAAGGGTTACGTACCGCGA |