| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123523 |
| Chromosome: | chromosome 16 |
| Location: | 3486973 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674534 | (1 of 1) IPR012336//IPR013026//IPR013105//IPR019734 - Thioredoxin-like fold // Tetratricopeptide repeat-containing domain // Tetratricopeptide repeat 2 // Tetratricopeptide repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACACCATGCGCATGTTCCCACACCCAAT |
| Internal bar code: | GACGACGGCGCAAATTGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 99.7822 |
| LEAP-Seq n confirming: | 5497 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGGCTCTGCTGCCGTTAT |
| Suggested primer 2: | GGGTAAAGGGAGCATAAGCC |