| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123882 |
| Chromosome: | chromosome 4 |
| Location: | 3130674 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225900 | VAM2,VAMP72,VAMP2 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III); (1 of 1) PTHR21136//PTHR21136:SF73 - SNARE PROTEINS // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACATCCGCATTTTGCTCAACTAGGGCGC |
| Internal bar code: | GCGTGTGTTTGCACCTGAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 857 |
| LEAP-Seq percent confirming: | 99.6992 |
| LEAP-Seq n confirming: | 2983 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACTGCTACATTCTGCAT |
| Suggested primer 2: | AACGACCTTGAATCCTCACG |