Insertion junction: LMJ.RY0402.123896_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.123896
Chromosome:chromosome 3
Location:1116997
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g148950 CGL43,SRRP1,PNT1 Ortholog of PSII biogenesis protein SRRP1; (1 of 3) IPR003029//IPR012340//IPR022967 - S1 domain // Nucleic acid-binding, OB-fold // RNA-binding domain, S1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCGTGCTGCGAGTGCGAGGTCCACCCAT
Internal bar code:TGGAGTAATGCTGCCAGCCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:99.292
LEAP-Seq n confirming:1683
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGTTTTGTTTGGCAGGTC
Suggested primer 2:CCGAGACACCCTCTGAAGTC