| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.123999 |
| Chromosome: | chromosome 4 |
| Location: | 3132885 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g225900 | VAM2,VAMP72,VAMP2 | Endosomal R-SNARE protein, Vamp7/Nyv1-family (R.III); (1 of 1) PTHR21136//PTHR21136:SF73 - SNARE PROTEINS // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGTCGATGGTTCTTTGTTCTTTGCGT |
| Internal bar code: | GCGAATGGATGCCTCTACTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 99.5261 |
| LEAP-Seq n confirming: | 210 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGAGCAGTTCCAAAAGAAG |
| Suggested primer 2: | ACCCGGCCTAGATAGCCTAA |