Insertion junction: LMJ.RY0402.124028_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.124028
Chromosome:chromosome 3
Location:2306792
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g158250 SCY2,SECY2 (1 of 1) PTHR10906:SF2 - PREPROTEIN TRANSLOCASE SUBUNIT SCY2, CHLOROPLASTIC; Preprotein translocase secY subunit 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCATAAGTGTTGAGTGGACGAGGGTGAG
Internal bar code:AAAATGAATAAGGACAGTAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:805
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:468
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GGTACATCCTCCGTGCTGAT
Suggested primer 2:CCTGCTGCACAGTGTTGAGT