Insertion junction: LMJ.RY0402.124811_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.124811
Chromosome:chromosome 4
Location:676597
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGGTGGCACCAATGGCCACCTGGGGGGC
Internal bar code:GGGCGGCACACGGTAGGACTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:671
LEAP-Seq percent confirming:99.53
LEAP-Seq n confirming:847
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGACGGGATAAAGATGGA
Suggested primer 2:GACGCGGTTAGCTTTGACTC