| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.124896 |
| Chromosome: | chromosome 13 |
| Location: | 1248350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570500 | (1 of 2) PF13374//PF13424 - Tetratricopeptide repeat (TPR_10) // Tetratricopeptide repeat (TPR_12) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATGCTCTTTCTCAACACCGCCCATGCG |
| Internal bar code: | TCCTTCTGCCGCTGGACTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAAAAAGCGGTACGGGA |
| Suggested primer 2: | ACCGCTTTGTTTCTACCCCT |