Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.125488 |
Chromosome: | chromosome_14 |
Location: | 477549 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g611100 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGCTGGGATGGAATCCGTGCTGCACCGC |
Internal bar code: | GGCCCGCGACTAGCAATGTCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 836 |
LEAP-Seq percent confirming: | 96.6292 |
LEAP-Seq n confirming: | 86 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCGATAGCTCCTGTCAATG |
Suggested primer 2: | GGGTTAGGGGTTTGTTCCAT |