Insertion junction: LMJ.RY0402.125913_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.125913
Chromosome:chromosome 13
Location:2220037
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCCAATCAATCTCTTTCCAATCAAGCC
Internal bar code:GCCCCTTCTGTAACGTCAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:340
LEAP-Seq percent confirming:99.1643
LEAP-Seq n confirming:1424
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCTCCACACACACACAC
Suggested primer 2:GGCGGCAGAGTTAAGAACAC