| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126029 |
| Chromosome: | chromosome 12 |
| Location: | 3798214 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515350 | 3'UTR|CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCACAGATCAAAGACAGCGGTACCGCA |
| Internal bar code: | GCGTATAAACCAAGTCGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.8312 |
| LEAP-Seq n confirming: | 2365 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACCGAGAATTGTGCAG |
| Suggested primer 2: | GTCAGCATTCTTGACTGCCA |