| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126351 |
| Chromosome: | chromosome 16 |
| Location: | 2677189 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662150 | CPLD51,CCB1 | conserved protein required for cyt b6 assembly; (1 of 1) PF12046 - Protein of unknown function (DUF3529) (DUF3529) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTCCCGAAACAAGCCCGCTCGCACGGG |
| Internal bar code: | ACATATGGTGGGGTTTGCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 99.8493 |
| LEAP-Seq n confirming: | 3312 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTCCCAGAGCAGAGTTC |
| Suggested primer 2: | ACATCATCGTGGAGGGAGAC |