Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.126702 |
Chromosome: | chromosome_16 |
Location: | 1277765 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g651400 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGGGGGCTGCTGTTGAGCCCGTCCGCGGG |
Internal bar code: | CGGCTTAATAGCTTACCACGTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 327 |
LEAP-Seq percent confirming: | 99.8476 |
LEAP-Seq n confirming: | 5240 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGGCGGATGGGTTATAATG |
Suggested primer 2: | TAAGCTGTGGCTCTCGTGTG |