| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.126750 |
| Chromosome: | chromosome 3 |
| Location: | 4176585 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173800 | PDXK1,SOS4,PDX2 | (1 of 1) 2.7.1.35 - Pyridoxal kinase / Vitamin B6 kinase; Pyridoxal kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCGCGACCCGGAAACGAGTCGCCACG |
| Internal bar code: | ATGCATTTGTCGGGCCAAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 81.9577 |
| LEAP-Seq n confirming: | 7904 |
| LEAP-Seq n nonconfirming: | 1740 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGAGAGCTGCAGGGAGA |
| Suggested primer 2: | CTGACCGCACTCCTAAAAGC |