| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.126888 |
| Chromosome: | chromosome_5 |
| Location: | 2059777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234500 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGAATATGGGCCGGCGTCAAGCGCAAACGC |
| Internal bar code: | TCTCATTGCGGAGATGGCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCTGGGAGGTGGTGAGT |
| Suggested primer 2: | GCGCTGTCATCAACTTGAAA |