Insertion junction: LMJ.RY0402.127046_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.127046
Chromosome:chromosome 11
Location:1008040
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467665 (1 of 1) IPR002909//IPR006626//IPR011050//IPR014756//IPR019316 - IPT domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // Immunoglobulin E-set // G8 domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGATCTAGGATCTGGGACCTAGGATCT
Internal bar code:GTCTCAGGATAACAGCCCATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:783
LEAP-Seq percent confirming:99.8543
LEAP-Seq n confirming:1371
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCGTACAAGTTACGGTTT
Suggested primer 2:CACGTCAACGTACTCGCACT