| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.127089 |
| Chromosome: | chromosome 16 |
| Location: | 4169638 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668900 | A3,MTA3 | Hypothetical protein; (1 of 2) PTHR11266:SF41 - PXMP2/4 FAMILY PROTEIN 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGTAGTGGTCGTGCCTTGCAGCCTGTG |
| Internal bar code: | CGTTGGACTTATTCTTGAATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1051 |
| LEAP-Seq percent confirming: | 54.6875 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGCACGGATGAACAAAA |
| Suggested primer 2: | CGGAGTTTTCTGAAAGCAGG |