Insertion junction: LMJ.RY0402.127154_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.127154
Chromosome:chromosome 5
Location:1222665
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g245351 (1 of 1) PF00059//PF00530//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGGACCGCCTGACACCTCTCTGGTCAAG
Internal bar code:TTCAGATTCTAACAATATGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1103
LEAP-Seq percent confirming:99.1018
LEAP-Seq n confirming:331
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCATCCCTCTGTGATGTGTG
Suggested primer 2:CCTCCTCGCTAACCACAGAG