Insertion junction: LMJ.RY0402.128081_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.128081
Chromosome:chromosome 14
Location:3735607
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632050 POB25,RPG1,RPGRIP1 Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAGGAGCGTGTGGAGGTGCTTGAGGACG
Internal bar code:CACTGGGGGGAGGACATGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:751
LEAP-Seq percent confirming:99.8917
LEAP-Seq n confirming:2768
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTTGGTTTAATCTGGGCTGG
Suggested primer 2:CGGTACATCAAATGGCACAG