| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128170 |
| Chromosome: | chromosome 9 |
| Location: | 5832566 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402219 | LCI3 | Low-CO2-inducible protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTTTGTGTCTCTCAACCAGGAACCTTC |
| Internal bar code: | AATGATCCCCACTTTACTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.7638 |
| LEAP-Seq n confirming: | 1267 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCTCACTCCCTCAACAA |
| Suggested primer 2: | TTTCTCCTGATCCATCCCTG |