| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.128420 |
| Chromosome: | chromosome 3 |
| Location: | 8063567 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201250 | (1 of 1) PF01585//PF12171 - G-patch domain (G-patch) // Zinc-finger double-stranded RNA-binding (zf-C2H2_jaz) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCTAGCACGCAGGGCCGGCATGGGCAT |
| Internal bar code: | GATTCTAATAGATGTGGAAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAGAAGTCCGTGCACCT |
| Suggested primer 2: | ACGTATCAAGTCTGTGGGGC |