| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.128476 |
| Chromosome: | chromosome 4 |
| Location: | 144550 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g216850 | TUA2 | (1 of 2) K07374 - tubulin alpha (TUBA); Alpha tubulin 2 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGTCTGTTGGTGTCAACTATTAGCGTG |
| Internal bar code: | CTTAATACATGCACCGCCGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 98.5048 |
| LEAP-Seq n confirming: | 2240 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGCTGACACCAGATAG |
| Suggested primer 2: | ACACTCATGGGTCGTGAACA |