| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129100 |
| Chromosome: | chromosome 16 |
| Location: | 2595600 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661626 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCCAAATGATACTGCAAACGCCCTGCG |
| Internal bar code: | AAGTTTTCAGTATACAAGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1188 |
| LEAP-Seq percent confirming: | 99.5573 |
| LEAP-Seq n confirming: | 1799 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTAATTCTGGTGCCGTT |
| Suggested primer 2: | TTTCCAAACTTTACGCCCAC |