| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129255 |
| Chromosome: | chromosome_10 |
| Location: | 3198366 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g442600 | XUV5,UAPA2 | Xanthine/uracil/vitamin C permease-like | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGTCGTGTGTGTTCGGGGTTATGAATGCG |
| Internal bar code: | TTACCTCGTCAACCTTTGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 706 |
| LEAP-Seq percent confirming: | 77.8194 |
| LEAP-Seq n confirming: | 1456 |
| LEAP-Seq n nonconfirming: | 415 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGTCGGTCTAATAAACA |
| Suggested primer 2: | GTAGATGCCATGCAAGCTCA |