| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129258 |
| Chromosome: | chromosome_13 |
| Location: | 2133561 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g577950 | VPS60 | Subunit of the ESCRT-III complex | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCTGGCCGCGTCTTCTTGATTTGCTCCT |
| Internal bar code: | TTGAAGGTCACGATGCGATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 681 |
| LEAP-Seq percent confirming: | 97.3384 |
| LEAP-Seq n confirming: | 256 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAACACGACCACCAAGTA |
| Suggested primer 2: | CACATTCCCCACACTCACTG |