| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.129336 |
| Chromosome: | chromosome 12 |
| Location: | 694610 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494750 | PRPS20 | Chloroplast ribosomal protein S20; (1 of 1) K02968 - small subunit ribosomal protein S20 (RP-S20, rpsT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGCAGAGCAGGAGGCTGTGGCTCTGGC |
| Internal bar code: | AGACCGTCTGGCACCAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 99.8142 |
| LEAP-Seq n confirming: | 6984 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTCTATAACAAGTCGCA |
| Suggested primer 2: | CTCTCATAGACGTGTGCGGA |