| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.129354 |
| Chromosome: | chromosome 7 |
| Location: | 5289552 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349500 | (1 of 2) PTHR15574:SF40 - WD AND TETRATRICOPEPTIDE REPEATS PROTEIN 1 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTGGAAGCAACCGGTGTTGCATCACGGC |
| Internal bar code: | GCACCGATGGAGGGAACCCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.3143 |
| LEAP-Seq n confirming: | 869 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCGACCTGAAGGCTAGA |
| Suggested primer 2: | TCTGGGTCCTGCTTACCATC |