| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.129358 |
| Chromosome: | chromosome 5 |
| Location: | 446673 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232550 | GPM4,PGM4,CA1P1 | (1 of 1) 3.1.3.63 - 2-carboxy-D-arabinitol-1-phosphatase; 2-Carboxy-D-arabinitol-1-phosphatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTGTAAAAGGCGGGCTGTTGTGTCGT |
| Internal bar code: | TGGGCGGGAACAATGTCGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 535 |
| LEAP-Seq percent confirming: | 98.8889 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACGTGTACGGTGTTGAA |
| Suggested primer 2: | TGTTGTTGATACAGCGCACA |