Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.129521 |
Chromosome: | chromosome_14 |
Location: | 972419 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g613950 | ABC2 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGCAGTACACTGGAGGGTTTCATCTTGCAG |
Internal bar code: | CCGTCGTGCGTGATTTGGGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 806 |
LEAP-Seq percent confirming: | 99.8541 |
LEAP-Seq n confirming: | 4791 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACCAGACCTCACCTCACACC |
Suggested primer 2: | CGGGAAAAGTGGTTAGACGA |